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User 3 split his 500mcg dose as 250mcg every 12 hours specifically to account for what he cited as a 4 to 6-hour half-life

This causes an increase in glucose-dependent insulin secretion which results in a decrease in glucagon secretion (by stimulating the release of insulin and suppressing the release of glucagon, the body thinks it has enough insulin and the body will in return slow down glucagon production/release)

In human monocytes, which do not express TLR7, 2O-methylated RNA also significantly reduces TNF- and IL-12p40 expression, likely due to the absence of TLR8 activation

Nature 426 , 442446 (2003)