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Lastly, SPF is used, and the chemical peel process is complete

All experiments were independently repeated at least three times with similar results unless otherwise stated

The human GCLC expression plasmid was constructed using the Gateway Cloning system (Thermo Fisher Scientific) and the pCMV-SPORT6-GCLC vector containing a human GCLC cDNA clone (Horizon Discovery Catalog #: MHS6278-202759380, Clone ID: 6052162, NCBI GeneBank Accession: BC039894), the Gateway pDONR221 Vector (Thermo Fisher Scientific), and the pLenti-CMV-Neo-DEST (Addgene Plasmid #17392) destination vector