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Proteins on gel were transferred to polyvinyl difluoride (PVDF) membranes followed by blocking with 5% non-fat milk for 2 h

Cost of one vial of fat in injection is $50$800 however, multiple vials are needed for the full treatments, and sometimes in the single sessions, so total treatment for arms typically requires 26 sessions, bringing the full cost for both arms to roughly $800$3,900+, depending on the amount of fat and the provider

Comparator Selection: Full-length hGH (to demonstrate receptor-independent effects) -adrenergic agonists (to show distinct mechanism) Vehicle control 4