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Additionally, the qPCR data revealed that A2ARi significantly increased gene expression of gpx4 and gclc , and the addition of Lip-1 further resulted in increased expression of these GSH-related genes in the presence of A2ARi (Supplemental Figure 8, A and B)

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Bacteria were harvested by centrifugation and lysed by sonication in a buffer consisting of 50 mM Tris pH 7.5, 500 mM NaCl, 5 mM DTT, PMSF and DNase I