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PPM1K mediates metabolic disorder of branched-chain amino acid and regulates cerebral ischemia-reperfusion injury by activating ferroptosis in neurons

Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18

Positive reports came almost entirely from the group that discovered the peptide

Peptides are short chains of amino acids that act as signaling molecules, helping regulate metabolism, appetite, fat storage, inflammation, and tissue repair at a cellular level

The gene level expression was evaluated using reverse transcription-polymerase chain reaction and protein expression was studied using Western blot analysis