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These microbial changes were accompanied by improved estrous cyclicity and significantly reduced androgen levels (though not fully normalized) ( 7 Discussion Accumulating evidence suggests that gut microbiome dysbiosis is closely associated with the pathophysiology of polycystic ovary syndrome (PCOS)

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Transient transfections were performed using Lipofectamine Plus reagents (Invitrogen Corp., Carlsbad, CA, USA) according to the manufacturer's instruction

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The concentration of fondaparinux sodium in umbilical cord plasma was approximately 1/10th the level of fondaparinux sodium in maternal plasma