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Vitamin B12 (cobalamin) is an essential water-soluble vitamin that plays a crucial role in red blood cell formation, neurological function, and DNA synthesis

For the SILAC assay, -Lys -Arg synthetic medium (0.17% yeast nitrogen base without amino acids, 2% glucose, 1 mM alanine, 1 mM asparagine, 1 mM aspartic acid, 1 mM cysteine, 1 mM glutamic acid, 1 mM glutamine, 1 mM glycine, 1 mM methionine, 1 mM proline, 2 mM leucine, 2 mM serine, 2 mM threonine, 0.75 mM isoleucine, 0.75 mM phenylalanine, 0.5 mM tyrosine, 0.3 mM histidine, 0.4 mM tryptophan, 2.5 mM valine, 0.15 mM adenine, 0.2 mM uracil) is supplemented with 30 mg/L L-lysine- 2 HCl (Thermo Scientific, 89987) or L-lysine- 2 HCl, 4,4,5,5-D4 (Thermo Scientific, 88437) and 5 mg/L L-arginine-HCl (Thermo Scientific, 89989) or L-arginine-HCl, 13 C 6 (Thermo Scientific, 88210)

B12 plays a role in producing mood-regulating neurotransmitters, helping to alleviate symptoms of stress, anxiety, and depression

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