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Administration of an antibiotic cocktail including ampicillin, metronidazole, and vancomycin, was found to disrupt ZO morphology while decreasing the expression of occludin and claudin-1 TJ proteins in mice (Feng et al

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This is true for all weight loss methods, but particularly relevant with GLP-1 medications because the appetite suppression is so effective that severe caloric deficits can occur