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Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based quantitative methods are one of the most commonly used techniques, with the advantage of being able to efficiently separate and detect a variety of carnitine and its derivatives, including free carnitine, acylcarnitine, and their isomers and odd forms

After blocked with 3% BSA in Tris-HCl (50 mmol/L Tris, pH 7.4, 200 mmol/L NaCl) buffer solution containing 0.1% Tween-20 for 4 h at room temperature, the membranes were incubated overnight at 4 C with specific antibodies against protein kinase B (AKT

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Microbiome 10 , 94 (2022)

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