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Epigenetic Drugs Limitations There are several drugs that modulate DNA methylation

doi: 10.1016/j.bpj.2017.08.050, 145

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Constructs were cloned into a pET21a vector (MilliporeSigma, Burlington, MA) using Gibson Assembly cloning 66 , and plasmids transformed into Rosetta2 cells (MilliporeSigma, Burlington, MA) or BL21 cells (Invitrogen, Carlsbad, CA)