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Subsequently, 300 L lysosomal-red fluorescent probe Lyso-Tracker Red working solution was added to the cells, and cells were further incubated in for an additional 50 min

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Six 40-second sprints have been shown to increase plasma BDNF four to five times compared to low-intensity activity

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7 Therapeutic implications and future directions At present, there is no curative or approved disease-specific treatment for ME/CFS, and clinical management remains largely supportive, with the primary goals of symptom relief and functional maintenance