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Protein pellets were resuspended by sonication (Sonics & Materials, VCX 130

This impaired clearance would also be compounded by exogenous copper downregulating LRP1 expression (Singh et al., 2013), that could further impede CNS clearance of LRP1 ligands such as Tau or A

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Phosphopeptides were eluted in three rounds with 80 l 1% ammonia for 20 min, transfered the supernatant to a clean plate, and acidified with TFA

yellow asterisk in (figure 2.13) and CRYAB-HIS (blue asterisk in figure 2.13)