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Similarly, Raddeanin A enhances mtDNA release and activates cGAS-STING and NF-B pathways, increasing cytokine secretion and tumor immunogenicity

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Membranes were blocked in PBS 0.1% Tween 3% Nonfat milk (Applichem, #APA08301000) and incubated with the appropriate primary antibodies diluted in PBS-0.1% Tween-3% Nonfat milk at 4 C overnight