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PDO image analysis For analysis of PDO size, images were captured under a light microscope and processed and quantified using Open Source Computer Vision Library ( The images were processed using a python script (Supplemental Fig

Although different cryopreservation methods are discussed separately here, it is essential to integrate a rational scientific mechanism with multidisciplinary techniques to translate cold tolerance strategies into cryopreservation practices for biosamples

Wilkins et al., 2012), mainly in kidney and liver, and tricarboxylate carrier (TTC, SLC25A1) in brain mitochondria and astrocytes (Wadey et al., 2009)

This dual-action strategy is many peoples preferred way to maintain optimal levels, especially during times of high stress or seasonal changes