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The homogenate was centrifuged at 17,600 g for 10 min at 4 C, and the supernatant was transferred to a new tube containing CaCl 2 (500 M) and immunoprecipitated with anti-HA agarose (Sigma-Aldrich)

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160,658,659 However, it is essential to interpret zinc effects cautiously and assess their physiological relevance in such studies

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Its important to know the differences to treat patients well