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Mello, M.L.S

In our study, as the intracellular -GC level was only increased slightly compared with that of original strain W303-1b when F pathway was introduced, we artificially introduced a flexible six-glycine linker between the two enzymes of G pathway as well as P pathway to improve the coupling efficiency by mimicking the native domain fusion of GshF

The full nrf2a coding sequence fused to the mKO2 protein via the T2A peptide was synthesized by Genewiz Company and was subsequently cloned into a pME vector to create a pME- nrf2a _T2A_mKO2nls vector