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Dots show individual samples, with R 2 indicated on the y-axis

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Cells were loaded with MitoTracker Red dye (Excitation/Emission: 579/599 nm) at 100 nM final concentration (37 C for 15 min) mounted in Live Cell Imaging Solution (Thermo Fisher), and viewed under a laser confocal microscope (LSM 510

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Research has recognized ferroptosis as a possible pathogenic mechanism associated with lumbar disc herniation, although detailed mechanistic investigations remain scarce