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Wang Y, Yang Z, Zheng G et al (2019) Metformin promotes autophagy in ischemia/reperfusion myocardium via cytoplasmic AMPK1 and nuclear AMPK2 pathways

doi: 10.1016/0076-6879(87)47121-8

Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C