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Overexposure of plants to Sub stress typically results in hypoxia, shortage of oxygen, or anoxia, a total absence of oxygen, which ultimately leads to tissue damage or plant death in the worst case (Loreti and Striker, 2020)

Shaking introduces shearing forces that can physically break the peptide bonds or cause the molecule to denaturerendering it completely useless

The volume of water you add directly determines the concentration (in mcg/mL) of your solution

doi:10.1002/ptr.6959 Rahimlou M, Asadi M, Banaei Jahromi N, Mansoori A

The PTK hydrogel-treated group showed the highest ICRS macroscopic score of 11.3 (the score was 12 for native cartilage), which was 2.9 and 5.7 times that in the PMI hydrogel-treated group and the control group, respectively