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Total RNA was extracted using TRIzol (Invitrogen, California, USA) and isolated using Oligotex mRNA Mini kit (Qiagen, Duesseldorf, Germany) according to the manufacturers instructions

1a) can be subdivided into a reductive half-reaction (electrons flow from NADPH to the enzyme) and an oxidative half-reaction (electrons from the enzyme are transferred to the oxidizing substrates) 17

Combined, these data suggest very low occupancy of Ca 2+ -binding sites in full-length Nedd4-2

The relatively low levels of recreational exercise among women in China, compared to other countries, may explain why a similar protective effect was not observed between non-occupational activity and UFs [30]