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Fini ME, Cook JR, Mohan R

Trypan blue exclusion viability assay Cells were seeded at 4 10 5 cells/well in 6-well plates and allowed to adhere overnight

We induced APAP hepatotoxicity in mice either sufficient of serotonin (wild-type mice and TPH1-/- plus 5- Hydroxytryptophan (5-HTP)) or lacking peripheral serotonin ( Tph1-/- and wild-type mice plus p-chlorophenylalanine (PCPA)).Mice with sufficient 5-HT exposed to acetaminophen have a significantly lower mortality rate and a better outcome compared with mice deficient of 5-HT