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This could explain why the activity of rhGPx3 mutant using H 2 O 2 as oxidizing substrate was in the same order of magnitude as that of human native GPx4 (34.8 U/mg) 9 and GPx4 mutant with all Cys converted to Ser (14.0 U/mg) 16 , while it was much lower than that of native GPx4 (64.6 U/mg) and the GPx4 mutant (24.6 U/mg) in the case of using PLPC-OOH as the oxidizing substrate

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These results show the protective effect of TCF21 and SMC phenotypic regulation in CAD (169)

Thus the redox potentials of GSH and other thiols can only be determined indirectly by measurement of equilibrium constants for their reaction with redox systems of known redox potentials 7