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Phase I-III Metabolic Detoxification Cascade Detoxification proceeds via three enzymatic phases: Phase I enzymes (cytochrome P450 oxidases) introduce reactive groups to xenobiotics

For a 2.5 mg target dose: Volume to Draw = Desired DoseConcentration = 2.5 mg10 mg/mL = 0.25 mL Convert to syringe units

Following, the cell pellets, containing a known number of cells (~ 1 10 6 cells/mL), were dissolved in 1 mL reagent: 1 N sodium hydroxide (NaOH) and 10% DMSO, and boiled at 60 C for 30 min

Wait 24 hours and check for any unusual reaction

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