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178 In addition, the secreted Cu-binding glycoprotein SPARC (secreted protein acidic and cysteine-rich, also known as osteonectin) has been shown to modulate cellmatrix interactions and promote the invasion and metastasis of tumor cells

Lysates were clarified by centrifugation at 16,000 g for 20 min at 4 C, and protein concentrations were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific)

The detoxification process in the body removes toxins, waste products, and chemical stressors

doi: 10.1111/nbu.12334 110

Inhibition of cystine input inhibits the synthesis of GSH in cells to the large extent and induces ferroptosis