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Cells were prepared by trypsinization and washed twice with PBS, then stained with 1 ml of DNA staining solution and 10 L Permeabilization solution

Diffusion tensor imaging (DTI) for white matter tract integrity

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When we carefully examined the profile of supersulphide metabolites with or without CARS2/CPERS KD using our sulphur metabolome, the amount of supersulphides was found to be decreased by approximately 30 M (Supplementary Fig