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Western blotting After treatment with bortezomib (4 nM), UroA (15 M), their combination, or vehicle (as a control), cells were lysed and cleared by centrifugation (14,000 g , 4 C, 5 min), and the protein concentration was determined with the Quick Start Bradford 1 Dye Reagent (Bio-Rad Laboratories, Hercules, CA, USA) on the Epoch microplate reader

Journal of renal nutrition: the official journal of the Council on Renal Nutrition of the National Kidney Foundation

In that respect, IM8 comes in at a sort of bargain, although its up-front price tag remains beyond reach for most people

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Cells were plated in 6-well plates at 7 10 5 cells per well and transfected with 2.5 g of the appropriate TCR or CAR construct in the retroviral vector pMP71 using Lipofectamine 3000 (Life Technologies, Invitrogen)