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In 1964, Robert Pecora developed the formalism showing that the diffusion of macromolecules in solution could cause a broadening of the frequency spectrum of scattered light, thereby laying a framework for interpreting experiments on Doppler shifts in light scattering from pure liquids and polymer solutions and indicating that Doppler shift measurements should yield information about the shape of the polymer molecule, and its translational and rotational diffusion coefficients and pointing out that the recently developed optical maser has made such experiments practical (Pecora, 1964)