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(1997), Hsu et al

After paper filtration, 100 L of sodium cyanide solution (1%, w/v) was added to the filtrate, and the filtrate was then shaken horizontally at 300 rpm (Shaker KL 2, Edmund Bhler, Bodelshausen, Germany) for 10 min under normal laboratory light conditions

NAR Cancer 5 , zcad049 (2023)

(Beijing, CN) and placed in specific pathogen-free environment at 2325 C with12/12 h light/dark cycle, offering regular diet

This timing ensures that the massive spike in serum melatonin coincides with the time you are trying to initiate sleep, maximizing its chronobiotic benefits while allowing the hormone to exert its antioxidant effects throughout the night during the body's natural repair phase