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RNA-Seq and enrichment analysis For oocytes, after denuding the excess cumulus cells and zona pellucida, 10 oocytes were collected for each sample, and the full-length mRNA was extracted by using Single Cell Full Length mRNA-Amplification Kit (Vazyme, Nanjing, China)

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Engineering a high-affinity PD-1 peptide for optimized Immune cellmediated tumor therapy