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For transformation, 3 l of the assembly product was used to transform 65 l of T7 Express chemically competent cells (NEB, no

Br J Oral Surg

Metabolic Regulation: Restoration of SCFA production, particularly butyrate, activates G-protein-coupled receptors (GPR41/43), inhibits the NF-B signaling pathway, and reduces the release of pro-inflammatory cytokines such as IL-6 and TNF- (Siddiqui and Cresci, 2021

This transition provides a critical mechanistic link between senescence-driven susceptibility and the emergence of widespread neuronal and synaptic pathology discussed in the following section