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Cells at passage 3 were used for downstream experiments

It has been reported that EGLN2 (Egl-9 family hypoxia inducible factor 2) is a candidate driver of iron chelation-mediated inhibition of cell death, and the hydroxylase activity of EGLNs is iron-dependent[114, 115]

Color development was performed using a DAB substrate kit, and the reaction was monitored microscopically and stopped when optimal signal-to-background contrast was achieved