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In Figure 4 It was observed that, compared to the normal architecture and regular pattern of hepatocytes with active vesicular nuclei in G1 (Normal control), the liver of G2 (MTX) showed central vein dilation and congestion (a in Figure 4B), Focal cell necrosis, hepatocyte nuclei of many cells are dark stained, and small apoptosis was also observed

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Prolonged oxidative stress has been linked to various pathological conditions, such as chronic inflammation, neurodegenerative diseases, cardiovascular disorders, and cancer [15,16,17]

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