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Food Funct 5(2):189197

The WT H1.4 coding sequence was amplified using primers 5- GGTTCTGGTGGTGGTTCGATGTCCGAGACTGCGCCT and 5- GGTACCGTCGACTGCAGCTACTTTTTCTTGGCTGCC and cloned into EcoRI-linearized C1L by Gibson assembly

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In brief, lipidomics for each sample were performed by the Vanquish UPLC systems coupled to a Q Exactive HF (QE-HF) mass spectrometer equipped with HESI (Thermo Fisher Scientific, Waltham, MA)