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To prepare the Transwell assay, murine melanoma cells (1 10 5 cells per insert) cultured in 200 l RPMI1640 medium supplemented with 0.5% FBS were plated on the upper insert (6.5 mm diameter transwell with 8 m pores, Corning), while 800 l RPMI1640 medium supplemented with 0.5% FBS, with or without hHGF or dimeric macrocycle was added to the bottom chamber

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These findings highlight the potential value of targeting GOT2 for alleviating cancer-induced immunosuppression [162]

doi: 10.1530/ERC130043 270 KinemanRDKamegaiJFrohmanLA

Two proteins play central roles in this process: collagen, which provides tensile strength and structure, and elastin, which allows skin to snap back after stretching