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Fixed cells were incubated with primary antibodies overnight at 4 C

Radiocarbon 62 , 725757 (2020)

In contrast, while salicylate-induced tinnitus models show significant astrocyte and microglial activation in the AC and medial geniculate body, including upregulation of GFAP and Iba1 alongside increased IL-1 expression (Xia et al., 2020), the causal dependence on microglial activation in this model remains less certainsome evidence suggests that salicylate-induced tinnitus may persist even when microglial activation is pharmacologically suppressed (Glia, 2015)

Compared to the large volume of literature regarding the impact of ROS on muscle fatigue, evidence that NO production directly contributes to the development of muscle fatigue is sparse

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