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Previous studies with MK-0677 in hip fracture patients suggested possible benefits to functional recovery

It can increase endogenous GH and IGF-1 to levels similar to those observed with exogenous GH therapy without exceeding physiologic norms [4]

Given that almost identical IC50 values for MTX were obtained by MTT and calcein AM at 48 and 96 hours, either one or the other method was used to access cell viability, depending on experimental and laboratory logistics