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The anti-inflammatory effect produced by IL-10 is partly through a negative feedback loop with astrocytes, where the binding of IL-10 causes the release of TGF- from astrocytes, which subsequently attenuates pro-inflammatory cytokine production in primary microglia cells (Norden et al., 2014)

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In a cell, equilibrium would be reached before these concentrations reached 0, because reactions with very low substrate concentrations do not follow convenience kinetics as simplified in the model