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For aldehyde detection using the Alexa Fluor 568 Hydrazide aldehyde-reactive probe (Life Technologies), TIS cells induced as described above were loaded with the probe via pinocytosis

It is noteworthy that during the initial phase of functional impairment in certain organs, such as liver injury, kidney injury, and cardiovascular disease, increased intestinal permeability and endotoxin release can be detected, which helps to identify some high-risk adverse events early (Lee et al., 2018)