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Plasma was collected at timepoints 0, 15 and 60 min by tail vein blood sampling and insulin concentrations were measured by enzyme-linked immunosorbent assay (ELISA

The kit used a unique molecular identifier (UMI) of 8 random bases to label pre-amplified cDNA molecules, thereby removing repetitive bias in PCR and sequencing steps

Clinical trials, such as BLISS-52 and BLISS-76, demonstrated that Belimumab decreases disease activity and flare rates, improving clinical outcomes in patients with SLE (126)

Subsequently, NanoSU6668 (ICG), MNS (ICG) and T-MNS (ICG) (25 M) were added to the culture medium and incubated for an additional 6 h