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Sekizawa, National Institute of Health Sciences, Tokyo, Japan Dr P

Data analysis was performed using FlowJo v10.9 (BD Biosciences)

Discarding the upper plasma, wash with three times volume of PBS for 3 times (re-suspend blood cell with PBS, centrifuge at 600 g for 10 minutes), add equal volume of Reagent I

Clin Chem 44:14231429 Fotakis G, Timbrell JA (2006) In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride

Fold changes were calculated relative to the AL group and log transformed subsequently for visualization in the heatmap