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To determine whether GSH related proteins linked to NFR2 regulated transcription showed time of day differences in their expression patterns, we examined the expression of Glutamate-Cysteine Ligase (GCLC) -the enzyme involved in the first step of GSH synthesis, Glutathione Synthetase (GS) - the enzyme involved in the second step of GSH synthesis, and Glutathione Reductase (GR) -the enzyme involved in the regeneration of GSH ( Figures 4BD )

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Lysosomes (Lys) and mitochondria (Mito)

doi:10.1038/s41586-019-1794-y