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y pET 15b y pET19b (NOVAGEN, Madison, WI), que contiene una secuencia lder His-Tag para su uso en la purificacin, que es una columna His y un sitio de escisin de protrombina que permite la escisin tras la purificacin en la columna, la regin del promotor T7-lac y el terminador T7.In a specific embodiment, a promoter-containing vector is used operably linked to nucleic acids encoding an FX polypeptide or a modified FX polypeptide, or a domain, fragment, derivative or homologue thereof, one or more origins of the replication and, optionally, one or more selectable markers (eg, an antibiotic resistance gene)

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M.HatfieldD

10.1016/j.bmc.2016.06.030 83 YokoyamaT.KitakamiR.MizuguchiM

The mitochondrial thioredoxin system