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Then, 400 L of 0.5% NaNO 2 (HiMedia, Mumbai, India) and 400 L of 0.5% AlCl 3 (Molychem, Mumbai, India) were added sequentially, with mixing after each addition

Initial inhibitor screens were conducted in 200 l volumes in 96 well plates with 50 M inhibitor, 100 M NADPH, and 5 nM TGR in 0.1 M potassium phosphate (pH 7.4), 10 mM EDTA with a preincubation step of 15 min

Calreticulin, an endoplasmic reticulum chaperone, is translocated to the cell surface as part of the unfolded protein response in stressed cells as a pre-apoptotic event (Obeid et al., 2007b)

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