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10.3389/fncel.2023.1328269 Front

Embo J 41(7):e109169

This limitation arises from the use of the ESI-MS/MS technique based on the in-solution buffer-free digestion method employed in this study to characterize RBD variants, which is not currently quantitative for such analysis ( In short, S-alkylation of C538 in the RBD protein leads to multiple chemical modifications resulting from free cysteines being exposed