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Description

VACV secretes approximately 100 immunomodulatory proteins that neutralise cytosolic nucleic acid sensors such as cGAMP synthase, along with several other antiviral response pathways 39

Rylander R, Tallheden T, Vormann J

The Cauchy pressure ( C 12 -C 44 ), an indicator of bond character and elastic behavior, is positive for all materials, implying ionic bonding and further supporting their ductile nature

After washing the resin repeatedly with kinase buffer (wash fractions), the protein of interest was eluted using 20 mM glutathione (Bio Basics Canada) and concentrated by centrifugation using 15 kDa Centrifugal Filter Units (Millipore)