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Measurement of glutathione levels

This is considered to be the rate limiting step because it is dependent on the bioavailability of cysteine and the activity of GCL, the latter of which is modified by competitive inhibition by reduced glutathione (GSH) [3134]

doi: 10.1002/mrm.21041 15 Baltazar-DazT

This decline is due to neuronal loss, formation of neurofibrillary tangles (NFTs) and amyloid plaques, ultimately resulting in neuronal death and tissue atrophy (155)