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The plate was incubated at 37 C for 5 days after which 20 L of PrestoBlue (Thermo) was added to each well, followed by an additional incubation of 2 days to allow the development of color

All materials and solvents used in the study were of analytical grade and were utilized without further purification

Pain, swelling, skin changes, ulceration in severe cases

Additionally, we show that CAR DNA binding is impaired, and we propose that HNF4 may regulate chromatin accessibility of CAR binding sites in sepsis