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Conclusion The carnivore diet is a subject of growing interest and debate within nutritional science

Cell culture The human ovarian granulosa cell line KGN (Procell, Wuhan, China) was cultured in Dulbecco's modified Eagle's medium (DMEM)-F12 medium (GIBCO-BRL, Invitrogen) containing 10% fetal bovine serum (Invitrogen) and 1% penicillinstreptomycin (Invitrogen) at 37C under 5% CO

The opening of mPTP, a multiprotein complex on the inner mitochondrial membrane, leads to a loss of transmembrane potential in and out of the mitochondria, which in turn causes mitochondrial swelling and rupture of the outer membrane [52]

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