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[DOI] [PubMed] [Google Scholar] Bai Y

Briefly, cells were lysed in Squash buffer (10 mM Tris pH8.0, 1 mM EDTA, 25 mM NaCl, supplemented with 200 g/ml Proteinase K) and heated in a Thermal Cycler (65 C for 15 min, 96 C for 2 min, 65 C for 4 min, 96 C for 1 min, 65 C for 1 min, 96 C for 30 s)

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Additional Information Background GSH-Px is one of the main enzymes that catalyze the oxidation of reduced glutathione (GSH) in the glutathione redox cycle