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However, our previous study revealed that fusion-expressed multivalent nanobodies exhibited reduced affinity, possibly due to the spatial structure blocking the binding epitope 13

Human monocytes THP-1 (CL-0233, Procell) were cultured in RPMI-1640 medium supplemented with 10% FBS, 0.05 mM -mercaptoethanol (M8210, Solarbio, Beijing, China), and 1% PSS

However, these methodologies are often multistep procedures and present different shortcomings such as non-specific adsorption, leakage, and/or partial denaturation of immobilized biomolecules [616,617]